MCQs – Biotechnology

1. Genetic engineering is the manipulation of ________ for practical purpose.

• Genetic bacteria
• Genetic plant
• Genetic material
• Genetic animal

2. The ________ technology has launched an industrial revolution in bio-technology.

• DNA transfer
• DNA recombinant
• genetic bacteria
• genetic transferring

3. Recent reproductive cloning intended to introduce ________.

• identical plants
• genetic bacteria
• identical animals
• identical sheeps

4. ________ was the first ever cloned animal.

• Dolly
• Tomy
• Kitty
• Spike

5. ________ formulated rule to explain the phenomenon of inheritance.

• Gregor Johann Mendel
• Charles Darwin
• Jean Baptiste de Lamark
• All of them

6. Each basis inheritable character is controlled by a factor called ________.

• Chromosome
• DNA
• RNA
• gene

7. Gene is made up of ________.

• Proteins
• RNA
• DNA
• All of them

8. The discovery of ________ was a tremendous stimulus to genetic research.

• DNA of bacteria
• cloned Dolly
• Role of DNA
• genetic engineering

9. Methodology was discovered in ________.

• 1952
• 1956
• 1973
• 1989

10. Methodological methods were referd to as ________.

• Recombinant DNA technology
• Genetic engineering
• Both
• All of them

11. ________ is defined as the “se of living organisms for the welfare of mankind”.

• Micro-biology
• Human biology
• Bbiotechnology
• Zoology

12. Genetic engineering usually utilizes ________.

• Bacterial cells
• Bacterial plasmids
• Both of them
• None of them

13. Recombinant DNA technology depends on ________ steps.

• 3
• 4
• 5
• 8

14. A gene is inserted into a DNA molecule called ________.

• vector
• pasmid
• both
• none of them

15. ________ are small, extra circular DNAs molecules found in some bacteria.

• vector
• plasmid
• chromosome
• genetic engineered DNA

16. Plasmid replicated ________ of the host cell.

• dependent
• independent
• mostly dependent
• mostly independent

17. Plasmids are generally found in ________.

• bacteria
• vertebrates
• all living organisms
• bacteriophages

18. The group of enzymes that are used to cut up the DNA molecule are called ________.

• restriction enzymes
• DNA ligase enzyme
• DNA polymerase enzyme
• none of them

19. ________ protest bacteria against bacteriophage by cutting viral DNA.

• DNA polymerase enzyme
• restriction enzyme
• DNA ligase enzyme
• all of them

20. The molecular scissors in the bacterial cell are ________.

• DNA ligase enzyme
• vector
• plasmid
• restriction enzyme

21. Each restriction enzyme cut the DNA at a special site called ________.

• sticky end
• site of attachment
• cutting end
• joining ends

22. ________ is a key enzyme that seals the restriction fragment with sticky ends of vector.

• DNA polymerase enzyme
• DNA ligase enzyme
• Restriction enzyme
• A and B

23. ________ are taken as host in DNA recombinant technology.

• bacterial cells
• vectors
• plasmids
• bacteriophage

24. The gene carried by recombinant molecule is called ________.

• cloned
• copied
• multiplied
• engineered

25. ________ is used to form genetically engineered plants which are resistant towards insects.

• genetically engineered bacteria
• vector
• plasmid
• all of them

26. Bacteria which usually live in the roots of corn are transformed for producing ________.

• good roots
• strong barks
• insect killing foxins
• healthy fruits

27. Hormones can also be prepared by ________.

• cloned bacteria
• plasmid
• vector
• rDNA

28. Phynylalanine is used to make a sweetener called ________.

• phenyl sweets
• nutra sweets
• cemilky sweets
• natural sweets

29. When a foreign gene is inserted to natural plants they are called ________.

• Transgenic plants
• genetically engineered plants
• both A and B
• all of them

30. In ________ technique entire organism can be grown from a single call.

• tissue culture
• cloning
• genetic engineering
• transfusion

31. When the cell wall of a plant cell is removed it is called ________.

• protoplast
• plant cell
• changed cell
• transgenic cell

32. In plant cell the rDNA can be introduced into ________.

• Enbryo
• Protoplast
• Cell having cell wall
• Both A and B

33. The only plasmid for transgenic plants is ________.

• Pr-plasmid
• Ti-plasmid
• Ds-plasmid
• All of them

34. ________ is the host for the host for the Ti-plasmid.

• Bacteriophage
• Agrobacterium
• Phenylalamine
• All bacteria

35. Transgenic plants are able to make use of ________ of the atmosphere so they dont require fertilizers.

• Oxygen
• Nitrogen
• CO2
• All of them

36. ________ is the fastest way of determining amino acid sequences.

• DNA finger printing
• Gene sequencing
• Genomic library
• Human genome project

37. ________ provides the location of Restriction site within the gene.

• Gene sequencing
• DNA finger printing
• Genomic library
• All of them

38. The similarity in the DNAs of two different organisms can be determined and studied by ________.

• DNA finger printing
• Human genome project
• Gene sequencing
• Gene therapy

39. ________ can separate DNA fragments differing in as much as 1 segment in gene sequencing.

• Gel electrophoreses
• DNA electrophoreses
• Gene electrophoreses
• All of them

40. ________% of DNA does not code for proteins

• 20%
• 30%
• 40%
• 50%

41. The difference in DNA electrophoresis patterns among individuals is called ________.

• DNA finger prints
• DNA fragment length
• DNA length measurement
• Restriction fragment length polymorphism

42. DNA finger prints is named so due to ________

• RFLPs length
• Sequence of RFLPs is unique for each
• Both A and B
• None of the above

43. To produce a DNA finger print ________ would suffice.

• A sample of blood
• A sample of saliva
• Sample of hair folicle
• All of them

44. ________ consists of the copy of genetic information or a specre the prefered environment.

• Genome library
• DNA finger prints
• DNA fragment
• Gene sequencing

45. Human Genome Project began in ________.

• 1990
• 1980
• 1994
• 1991

46. Total number of chromosome pairs inhuman is ________.

• 23
• 26
• 44
• 46

47. ________% of the DNA sequence have determined with 99% accuracy.

• 89%
• 95.658%
• 99.335%
• 99.99%

48. ________% of the nucleotide sequence in all the hamans is same.

• 50%
• 86%
• 99%
• 99.9%

49. Medical researches have discovered about ________ genetic diseases.

• 3000
• 4000
• 5000
• 6000

50. Genetic diseases is mostly related with ________.

• Sperms
• Ova
• Fertilized egg or zygote
• All of them

51. There are ________ conditions for the occurance of genetic diseases.

• 2
• 3
• 5
• 9

52. Is mutation a cause of genetic diseases?

• Yes always
• No
• May be
• Not involved at all

53. Genetic diseases fall into ________ main categories.

• 2
• 3
• 5
• 8

54. Following is the category of genetic diseases.

• Chromosomal abnormalities
• Unifactorial defects
• Multifactorial defects
• All of them

55. Huntington’s disease is due to a ________.

• Autosomal resesive allele
• Autosomal dominant allele
• Hetyerozygous allele
• Homozyhous allele

56. In huntington’s disease the symptoms appear by the age of ________.

• 35-45
• 40-50
• 55-65
• 50-60

57. Progressive mental deterioration and unvoluntary muscle movement is the characteristic of ________.

• Chromosomal disorder
• Chromosomal disjunction
• Huntington’s disease
• Cystic fibrosis

58. Is a cure for huntington’s disease possible.

• Yes
• May be
• Depends on nature
• No

59. Cystic fibrosis occurs due to ________

• Autosomal Recessive Allele
• A heterotroph
• Homozygous Chromosome
• Dominant Allele

60. ________ is a diagnostic process in which man amount of amniotic fluid is taken from the amniotic sac to determine the presence of disease.

• Genetic councelling
• Tissue culture
• Gene therapy
• Amniocentesis

61. Through ________ any disease can be determined in the embryonic stage.

• Amniocentesis
• Amniotic tissue test
• fefune blood test
• Gene therapy

62. ________ is a test tube method to create and olone various varieties of novel plants.

• Cloning
• Genetic engineering
• Gene therapy
• Tissue culture

63. The cell which contains all the genetic potential of the organism is called ________.

• Potitotent
• Totipotent
• Sturgid call
• Complete cell

64. Through cloning the duplicate coples of ________ can be formed.

• Genetic material
• Cells
• Both of them
• None of them

65. Artificial cloning is now possible by the pethology ________.

• Recombinant DNA technology
• Gene therapy
• DNA finger prints
• Genomic library

66. The copies of individuals formed by clonig are called ________.

• Twins
• Identicals
• Clones
• Copies

67. In 1997, a ________ was cloned from cell from its mother.

• Cat
• Dog
• Fish
• Sheep

68. The biotechnology is now also being considered dangerous due to

• Its useful cloning method
• DNA finger printing
• Harmful side effects
• Artificial Techniques

69. In gene therapy of germ line cells the recombinant DNA is inserted into ________.

• Human sex cells
• Fertilized zygote
• Somatic cells
• All of them